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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
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1Department of Medical Biotechnology and Translational Medicine,Università degli Studi di Milano, 2Department of Neuroscience; Department of Cell Biology,Howard Hughes Medical Institute, Yale University School of Medicine, 3Program in Cellular Neuroscience, Neurodegeneration and Repair,Yale University School of Medicine, 4Department of BioSciences,Università degli Studi di Milano
Capitoli
- 00:05Titolo
- 01:07pHuC_Mermaid Plasmid Generation
- 03:54Embryo Microinjection and Spontaneous Coiling Analysis
- 05:50Imaging Setup for Mermaid Biosensor Visualization in Living Embryos: Simulataneous Detection of Donor and Acceptor Signals
- 08:23Results: In Vivo FRET Analysis to Measure Changes in Motor Neuron Membrane Potential
- 09:39Conclusion
Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.
