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Using Primary Neurosphere Cultures to Study Primary Cilia
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JoVE Journal Biologia dello sviluppo
Using Primary Neurosphere Cultures to Study Primary Cilia

Using Primary Neurosphere Cultures to Study Primary Cilia

DOI:
10.3791/55315-v

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08:14 min

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April 14, 2017

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, , ,
1Department of Cell Biology,University of Texas Southwestern Medical Center

Capitoli

  • 00:05Titolo
  • 01:13Isolation of Neurospheres from the Adult Mouse Brain
  • 02:43Embedding and Sectioning of Neurospheres for immunostaining
  • 04:10Starvation and Analysis of Cilia
  • 06:01Results: Starvation Induces Ciliogenesis in Dissociated, Adherent Neutral Stem/Progenitor Cells
  • 07:19Conclusion

Summary

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The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.

Tags

Primary Neurosphere CulturesPrimary CiliaNeural Stem CellsNeural ProgenitorsNeuronsLateral VentricleSubventricular ZoneTrypsin-EDTABasal MediumNeurospheresImmunostainingParaformaldehydeSucrose

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