Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

Rivista

Biologia

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

DOI:

10.3791/55324-v

•

07:40 min

•

February 05, 2018

•

Aurelia C. Kucera, Britt J. Heidinger

¹Environmental and Conservation Sciences Program,North Dakota State University, ²Department of Biological Sciences,North Dakota State University

Capitoli

00:04Titolo
00:41Semen Collection from a Passerine Bird Using Cloacal Massage
03:34Extraction of DNA from Bird Semen
06:32Results: Success by Bird Species and DNA Electrophoresis
07:08Conclusion

Summary

Traduzione automatica

This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

•

•

•

Capitoli

Summary

Traduzione automatica

Tags

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Harvesting Sperm and Artificial Insemination of Mice

Isolation of Genomic DNA from Mouse Tails

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Isolation and Culture of Avian Embryonic Valvular Progenitor Cells

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment

DNA Electroporation, Isolation and Imaging of Myofibers

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Read Article