/

Biologia dello sviluppo

/

长期共焦成像根和下胚轴发育的简单室

JoVE Journal
Biologia dello sviluppo

This content is Free Access.

JoVE Journal Biologia dello sviluppo

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

Please note that all translations are automatically generated. Click here for the English version.

长期共焦成像根和下胚轴发育的简单室

DOI:

10.3791/55331-v

•

07:59 min

•

May 17, 2017

•

Charlotte Kirchhelle, Ian Moore

¹Department of Plant Sciences,University of Oxford

Capitoli

00:05Titolo
00:50Preparing Seedling Growth Imaging Chambers
04:01Using the Imaging Chambers
05:40Results: Root Growth in Chambers Compared to Agar Plates
07:15Conclusion

Summary

Traduzione automatica

这里介绍的是使用高数值孔径目标和全氟萘烷作为浸渍介质，用于高达3天的根和下胚轴发育的高分辨率共焦延时成像的简单技术。

Tags

Keywords: Confocal Microscopy Long-term Imaging Plant Development Root Hypocotyl Organogenesis Perfluorodecalin PDMS Agar Chamber Design

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

分析<em>在体内</em>细胞迁移使用细胞移植和时间推移成像在斑马鱼胚胎

配备了光学切片与成像定时使用解剖显微镜斑马鱼肾脏发展分析

水下滤纸三明治长期<em>防爆大毛</em>早期鸡胚时间推移成像

住发展拟南芥花的共焦成像

斑马鱼发展长期成像的多功能安装方法

长期的实时成像<em>果蝇</em>眼睛光盘

胚胎暴露于低剂量毒物的长期行为和生殖后果

不同植物种的射击顶端共聚焦活图

全山荧光在小鼠胚胎发生过程中心脏室发育的分析

半高通量成像方法和数据可视化工具包，用于分析C. elegans胚胎发育

Read Article