Rivista
/
Ricerca sul cancro
/
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

DOI:
10.3791/55357-v

•

06:19 min

•

July 18, 2017

•

, , , , , ,
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus,Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

Capitoli

  • 00:05Titolo
  • 00:40Orthotopic Mouse Model
  • 03:05Circulating Tumor Cell (CTC) Isolation from Whole Blood
  • 04:59Results: Representative Murine Colorectal Cancer Induction
  • 05:33Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

Tags

Circulating Tumor CellsOrthotopic Mouse ModelColorectal CancerMicrosurgical TechniqueCecal Wall InjectionReproducible Metastatic TumorsImmunocompromised MouseIntra-cecal InjectionMicro Injection PumpBinocular Surgical MicroscopeDensity Gradient MediumBlood Sample Collection

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Fluorescent Orthotopic Mouse Model of Pancreatic Cancer

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

Capture and Release of Viable Circulating Tumor Cells from Blood

A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma – An Improved Protocol

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

Read Article