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Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
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Neuroscienze
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JoVE Journal Neuroscienze
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

DOI:
10.3791/55359-v

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07:48 min

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August 27, 2017

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, , ,
1Department of Anatomy and Cell Biology,Martin Luther University Halle-Wittenberg, 2Department of Anatomy,University of Otago

Capitoli

  • 00:00Titolo
  • 00:08Agar Preparation
  • 00:54Brain Fixation
  • 02:29Slicing Procedure
  • 03:26Hippocampus Removal
  • 04:05Culturing Method
  • 05:18Representative Results
  • 07:19Conclusion

Summary

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Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.

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Organotypic Hippocampal Slice CulturesNeuroprotectionTumor Cell InvasivenessVibratomeAgar BlocksPreparation MediumCytoarchitectureCell Culture InsertCulture MediumInflammatory ReactionsNeuroprotection Studies

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