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En High-throughput Cell Microarray Platform for korrelative Analyse af Cell Differentiering og Traction Forces
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
DOI:

12:04 min

March 01, 2017

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Capitoli

  • 00:05Titolo
  • 01:05Fabrication of Polyacrylamide Substrates
  • 02:57Fabrication of Arrays
  • 05:15Cell Culture and Assay Readout
  • 09:06Results: Biochemical and Biophysical Cues in Liver Progenitor Differentiation
  • 10:58Conclusion

Summary

Traduzione automatica

Celledifferentiering er reguleret af en række microenvironmental faktorer, herunder både matrix sammensætning og substrat materialeegenskaber. Vi beskriver her en teknik under anvendelse af celle microarrays sammenholdt med trækkraft mikroskopi at evaluere både celledifferentiering og biomekaniske celle-substrat-interaktioner som funktion af microenvironmental kontekst.

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