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通过诱导瞬时转染可控离子通道表达
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Genetica
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JoVE Journal Genetica
Controllable Ion Channel Expression through Inducible Transient Transfection
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通过诱导瞬时转染可控离子通道表达

DOI:
10.3791/55370-v

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10:00 min

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February 17, 2017

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, ,
1The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine,The Hebrew University of Jerusalem (HUJI)

Capitoli

  • 00:05Titolo
  • 00:45Transfecting the Plasmid of Interest into Cells
  • 02:27Inducing Gene Expression
  • 03:48Calibrating Timeline of Protein Expression
  • 08:20Results: Response of TRPV1 Activation Increases According to Induction Time as Visualized through Calcium Imaging
  • 09:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

通过异源表达系统研究离子通道已成为生物医学研究核心技术。在这个手稿，我们提出了一个时间有效的方法通过诱导型启动子的控制下进行瞬时转染来实现紧密控制的离子通道的表达。

Tags

Inducible Transient TransfectionIon Channel ExpressionTREX 293 CellsTRPV1EGFPDoxycyclineElectrophysiologyCalcium ImagingPDL-coated Coverslips/chambersControlled Protein ExpressionHeterologous SystemsReplicable ResultsIon Channel ResearchPharmacology

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