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使用电刺激人干细胞衍生的心肌细胞的成熟中Biowires

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Maturation of Human Stem Cell-derived Cardiomyocytes in Biowires Using Electrical Stimulation

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使用电刺激人干细胞衍生的心肌细胞的成熟中Biowires

DOI:

10.3791/55373-v

•

10:11 min

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May 06, 2017

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Xuetao Sun, Sara S. Nunes^2,3,4

¹Toronto General Research Institute,University Health Network, ²Institute of Biomaterials and Biomedical Engineering,University of Toronto, ³Heart & Stroke/Richard Lewar Centre of Excellence,University of Toronto, ⁴Laboratory of Medicine and Pathobiology,University of Toronto

Capitoli

00:05Titolo
01:01Fabrication of Biowire Templates
02:22Fabricating the Electrical Simulation Chambers
03:55Gel Preparation and Seeding of Cardiomyocytes into the Template
05:41Culturing Cardiomyocytes in the Template with Electrical Stimulation (Biowire)
07:22Results: Biowire Cardiomyocyte Physiology
09:16Conclusion

Summary

Traduzione automatica

心脏biowire平台是使用三维细胞培养与电刺激结合成熟人胚胎和诱导的多能干细胞衍生的心肌细胞（HPSC-CM）的体外方法。这份手稿介绍了心脏biowire平台的详细设置。

Tags

Stem Cell Cardiomyocyte Maturation Electrical Stimulation Biowire Drug Screening PDMS Suture Polycarbonate Carbon Rod Platinum Wire

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