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Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method
 

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

Article DOI: 10.3791/55376-v • 16:07 min • September 16th, 2017
September 16th, 2017 •
1, 1, 1, 1, 1, 2
1Research and Development, Probiomed, 2Bioprocess Research and Development Unit, Instituto Politecnico Nacional

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A protocol for the determination of the relative anti-apoptotic activity of an anti-TNFα mAb using a neutralization mechanism with WEHI 164 cells is presented here. This protocol is useful for comparing the neutralization strength of different molecules with the same biological functionality.

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Relative Potency Anti-TNF Monoclonal Antibody Neutralizing TNF In Vitro Bioanalytical Method Reference MAb Sample MAb TNF-alpha Neutralization Potency Environmental Stress Factors Apoptosis Programmed Cell Death Anti-TNF Molecule Monoclonal Antibody Neutralization Process Cell Survival Standardized Methodology Cell Culturing WEHI 164 Cells Subculturing Confluency Of Cells
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