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Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
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Biologia
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JoVE Journal Biologia
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes

DOI:
10.3791/55378-v

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06:38 min

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November 22, 2017

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, , , ,
1Department of Biochemistry & Molecular Biology,Drexel University College of Medicine, 2Department of Obstetrics & Gynecology,Drexel University College of Medicine

Capitoli

  • 00:05Titolo
  • 00:52Spermatocyte Nuclei Spreading
  • 04:44Results: Representative Wild-type, Juvenile Mouse, Surface-Spread Nuclei
  • 05:32Conclusion

Summary

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Meiosis is the developmental process by which gametes are formed through a single round of DNA replication and two successive rounds of chromosome segregation. Mammalian meiosis can be examined by utilizing a technique to prepare meiotic chromosome spreads. Here, we demonstrate a method of preparing surface-spread nuclei from mouse spermatocytes.

Tags

Meiotic Chromosome SpreadsMouse SpermatocytesReproductive BiologyMeiotic RecombinationChromosome SegregationSeminiferous TubulesHypotonic Extraction BufferFixative SolutionTeflon Printed Slides

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