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分析树突状形态的列和图层

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Analyzing Dendritic Morphology in Columns and Layers

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分析树突状形态的列和图层

DOI:

10.3791/55410-v

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08:41 min

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March 23, 2017

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Chun-Yuan Ting, Philip G. McQueen, Nishith Pandya, Evan S. McCreedy, Matthew McAuliffe, Chi-Hon Lee

¹Section on Neuronal Connectivity, Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health (NIH), ²Mathematical and Statistical Computing Laboratory, Center for Information Technology,National Institutes of Health (NIH), ³Biomedical Imaging Research Services Section, Center for Information Technology,National Institutes of Health (NIH)

Capitoli

00:05Titolo
00:44Dual-image Acquisition
03:11Neurite Tracing and Reference Point Assignment
06:49Results: Comparison of Tm20 and Tm2 Neuron Morphology
08:04Conclusion

Summary

Traduzione automatica

在这里，我们将展示如何分析列和层果蝇髓质神经元树突路由。该工作流包括一个双视图成像技术，以改善跟踪，登记树突乔木于基准列数组以及用于分析在三维空间中的树枝状结构的图像质量和计算工具。

Tags

Keywords: Dendritic Morphology Drosophila Medulla Neurons Columns Layers Neuroscienze Dendritic Wiring Imaging Confocal Microscopy GFP Labeling Brain Clearing Sample Preparation

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