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基于β-酶的电导生物传感器检测技术在分子间相互作用中的应用

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Bioingegneria

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JoVE Journal Bioingegneria

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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基于β-酶的电导生物传感器检测技术在分子间相互作用中的应用

DOI:

10.3791/55414-v

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08:06 min

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February 01, 2018

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Marylène Vandevenne*¹, Mathieu Dondelinger*¹, Sami Yunus, Astrid Freischels, Régine Freischels, Oscar Crasson, Noureddine Rhazi, Pierre Bogaerts, Moreno Galleni, Patrice Filée

¹Life Science Department,University of Liège, ²Institute of Condensed Matter and Nanoscience,Catholic University of Louvain, ³Laboratory of Clinical Microbiology,Catholic University of Louvain, ⁴Biotechnology Department,CER Group

Capitoli

00:05Titolo
00:52Sensor Preparation and Regeneration
02:04Binding Assay Performed on the Sensor
04:45Results: Measurement of Conducticity Cganges Upon Antibiotic Hydrolysis by the Immobilization Chimeric β-Lactamase
06:35Conclusion

Summary

Traduzione automatica

在这项工作中, 我们报告了一个新的方法来研究蛋白质-蛋白质相互作用的电导生物传感器的基础上的混合β-酶技术。这种方法依赖于质子在β-内酰胺水解时的释放。

Tags

Beta-lactamase Conductimetric Biosensor Biomolecular Interactions Antibody-antigen Interactions Chimeric Beta-lactamase Proton Release Immunosensors Hybrid Protein Protein Sample Preparation Electrode Preparation Electrode Regeneration Hen-egg-white Lysozyme Polyaniline Phosphate Buffer Blocking Solution BlaP-cAb-Lys3 BlaP

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