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一个<em>体外</em鼠类的椎间盘>器官培养模型

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

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一个<em>体外</em鼠类的椎间盘>器官培养模型

DOI:

10.3791/55437-v

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08:03 min

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April 11, 2017

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Jennifer W. Liu³, Kevin H. Lin, Christian Weber, Sameer Bhalla, Sean Kelso, Kaixi Wang, Simon Y. Tang^3,4

¹Department of Biomedical Engineering,Washington University in St. Louis, ²Department of Biology,Washington University in St. Louis, ³Department of Orthopaedic Surgery,Washington University in St. Louis, ⁴Department of Materials Science and Mechanical Engineering,Washington University in St. Louis

Capitoli

00:05Titolo
00:57Animals and Dissection Protocol
02:47Organ Culture Conditions
04:11Longitudinal Measurements of NF-κB
04:39Mechanical Assessment
05:57Results: Properties of In Vitro-cultured IVDs
07:07Conclusion

Summary

Traduzione automatica

椎间盘（IVD）的整个器官培养保留天然的细胞外基质，细胞的表型，和细胞 - 基质相互作用。在这里我们将介绍使用鼠标腰部，并在其脊柱功能单位尾椎的IVD并利用该系统的几个应用的IVD文化体系。

Tags

Keywords: In Vitro Organ Culture Intervertebral Disc Murine Low Back Pain Disc Degeneration BALB/c Mice Transgenic Mice Luciferase Gene Functional Spine Units DMEM/F-12 Medium Organ Culture Method Dissection Sterility Contamination

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