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Immunologia e infezioni

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受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

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受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

DOI:

10.3791/55445-v

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07:46 min

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April 03, 2017

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Joselyn N. Allen, Adwitia Dey, Ruth Nissly, James Fraser, Shan Yu, Gayathri Balandaram, Jeffrey M. Peters, Pamela A. Hankey-Giblin

¹Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, ²Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Capitoli

00:05Titolo
00:51Liver Tissue Isolation
03:57Liver Cell Dissociation
05:23Results: Representative Liver-isolated Macrophage Characterization
06:59Conclusion

Summary

Traduzione automatica

该协议允许研究员分离和表征组织驻留巨噬细胞在各种标志发炎组织提取从饮食诱发的代谢紊乱模型。

Tags

Macrophage Isolation Perfusion Liver Obesity-related Inflammation Chronic Inflammation Immunology

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