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Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
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JoVE Journal Medicina
Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

DOI:
10.3791/55450-v

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06:49 min

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May 31, 2017

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, , ,
1Department of Translational Skin Cancer Research,University of Duisburg/Essen, 2Institute for Experimental Immunology and Imaging,University of Duisburg/Essen, 3Imaging Center Essen, Electron Microscopy Unit,University Hospital of Essen

Capitoli

  • 00:05Titolo
  • 00:56Diptheria Toxin (DPT) Myocarditis Induction and Light-sheet Microscope Sample Preparation
  • 02:12Light-sheet Microscopy and Image Post-processing
  • 05:16Results: Representative 3D Recordings of Whole Organ Light-sheet Microscopy
  • 05:58Conclusion

Summary

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Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.

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Keywords: Leukocyte InfiltrateMurine ModelFulminant MyocarditisLight Sheet MicroscopyWhole Organ VisualizationCardiac ArrhythmiaCellular ResolutionInflammation QuantificationCardiac ProsthesisFluorescence MicroscopyImage Processing

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