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时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
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Biochimica
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JoVE Journal Biochimica
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
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时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

DOI:
10.3791/55464-v

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09:18 min

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April 17, 2017

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, , , , ,
1Department of Chemistry,York University, 2The Centre for Research in Mass Spectrometry,York University, 3The Centre for Research on Biomolecular Interactions,York University

Capitoli

  • 00:05Titolo
  • 01:12Preparation of the Microfluidic Device
  • 05:17Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange
  • 06:51Results: Detection of Protein Structural Changes by Time-resolved ElectroSpray Ionization Hydrogen-deuterium Mass Spectrometry
  • 08:10Conclusion

Summary

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构象的灵活性在蛋白质功能的关键作用。在此，我们描述了使用耦合到氢 - 氘交换用于探测驱动功能在有序和无序的蛋白质的快速结构变化的时间分辨电喷雾电离质谱法。

Tags

Time-resolved Electrospray IonizationHydrogen-deuterium ExchangeMass SpectrometryProtein StructureProtein DynamicsConformational FlexibilityTransient Protein ConformationsLoop RegionsMolten GlobulesIntrinsically Disordered ProteinsNeurodegenerative DisordersProtein MisfoldingProtein AggregationEnzyme Catalytic TurnoverProtein-protein InteractionsProtein-ligand InteractionsContinuous FlowTime-resolved Kinetic MixerOrthogonal Mixing

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