Rivista
/
Biologia
/
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

DOI:
10.3791/55486-v

•

08:32 min

•

March 16, 2017

•

, , , , , , , , , , , , ,
1Institute of Molecular Biology and Biochemistry,Medical University of Graz

Capitoli

  • 00:05Titolo
  • 01:07Cell Preparation
  • 02:33Live Cell Imaging of NO• and Ca2+ Signals in Individual Cells
  • 06:28Results: Simultaneous Multichannel Imaging of NO• and Ca2+ Signals in Single EA.hy926 Cells
  • 07:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.

Tags

Genetically Encoded Fluorescent Nitric Oxide ProbesGeNOpsReal-time ImagingNitric Oxide SignalsSingle CellsFluorescence MicroscopeIron(II) Booster SolutionFura-2AMPerfusion ChamberGravity-driven PerfusionPhysiological Calcium Buffer

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Read Article