È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 00:54Lyse Bacterial Pellets Expressing Human Recombinant G4 Resolvase1 (rG4R1)
- 03:06Prepare G4-magnetic Beads
- 04:07Bind Histidine-tagged rG4R1 to Cobalt Beads
- 05:47Elute rG4R1 from Cobalt Beads
- 07:07Bind rG4R1 to G4-bound SPB and Elute in an ATP-dependent Manner
- 08:55Results: Analysis and Quantification of Catalytically Active, Purified rG4R1
- 10:06Conclusion
