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Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
JoVE Journal
Genetica
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JoVE Journal Genetica
Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
DOI:

10:59 min

May 24, 2017

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Capitoli

  • 00:05Titolo
  • 00:52Protein-DNA Sample Preparation for AFM
  • 03:14AFM Imaging of Protein-DNA Sample
  • 06:37AFM Analysis of Protein Complex Volume
  • 07:51Results: Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
  • 09:25Conclusion

Summary

Traduzione automatica

Here, the study of different DNA lesion recognition approaches via single molecule AFM imaging is demonstrated with the nucleotide excision repair system as an example. The procedures of DNA and protein sample preparations and experimental as well as analytical details for the AFM experiments are described.

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