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In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
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Capitoli
- 00:05Titolo
- 01:07GnRH Uptake
- 02:39Type I GnRH Receptor Expression
- 05:39Fluorescence Microscopy Imaging
- 06:26FACS-assisted GnRH Quantification
- 08:00Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells
- 09:25Conclusion
Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.
