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激光局部补骨脂素加合物的单分子分析
JoVE Journal
Genetica
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JoVE Journal Genetica
Single Molecule Analysis of Laser Localized Psoralen Adducts
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激光局部补骨脂素加合物的单分子分析

DOI:
10.3791/55541-v

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11:46 min

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April 20, 2017

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, , , , , ,
1Laboratory of Molecular Gerontology,National Institute on Aging, National Institutes of Health

Capitoli

  • 00:05Titolo
  • 00:53Calibrating the Targeting Laser
  • 04:54Laser Localized Dig-TMP ICLs
  • 07:44Harvesting Cells and Stretching DNA Fibers
  • 09:51Results: Laser Localized Psoralen Adducts Reveal the DNA Damage Response to ICLs
  • 11:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

激光器在对DNA损伤的细胞应答的研究经常使用的。然而，它们产生的病变，其间隔，频率，和碰撞与复制叉很少表征。在这里，我们描述了一种方法，使这些参数与激光局部间交联的决心。

Tags

Single Molecule AnalysisLaser Localized Psoralen AdductsDNA Interstrand CrosslinksDNA RepairLaser Activated CompoundMirror Slide CalibrationLaser TargetingBiological CalibrationTrimethylpsoralenGFP Tagged Repair FactorU2OS Cell Line

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