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In vitro- metoder til sammenligning af målbinding og CDC-induktion mellem terapeutiske antistoffer: applikationer i biosimilaritetsanalyse
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

In vitro- metoder til sammenligning af målbinding og CDC-induktion mellem terapeutiske antistoffer: applikationer i biosimilaritetsanalyse

DOI:
10.3791/55542-v

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07:25 min

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May 04, 2017

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, , , ,
1Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences,National Polytechnic Institute (IPN), University of Mexico (UNAM), 2School of Chemistry,National Autonomous University of Mexico (UNAM), 3Graduate Program in Chemical Sciences,National Autonomous University of Mexico (UNAM), 4Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences,National Polytechnic Institute (IPN), 5Department of Immunology, National Scool of Biological Sciences,National Polytechnic Institute (IPN), 6Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine,National Autonomous University of Mexico (UNAM)

Capitoli

  • 00:05Titolo
  • 00:48Flow Cytometric Target Binding Analysis
  • 03:35Complement-dependent Cytotoxicity (CDC) Assessment
  • 05:29Results: Representative Anti-CD20 Therapeutic Monoclonal Antibody Analyses
  • 06:38Conclusion

Summary

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Denne protokol beskriver in vitro sammenligning af to centrale funktionelle egenskaber af rituximab: targetbindende og komplement-afhængig cytotoksicitet (CDC) induktion. Metoderne blev anvendt til en side-til-side sammenligning mellem henvisning rituximab og en rituximab biosimilært. Disse assays kan anvendes under biosimilar udvikling eller som en kvalitetskontrol i deres produktion.

Tags

Therapeutic AntibodiesBiosimilarity AnalysisTarget BindingComplement-dependent Cytotoxicity (CDC) InductionIn Vitro MethodsRituximabDaudi CellsReference ProductBiosimilarQuality Control

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