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Capitoli
- 00:05Titolo
- 00:54Venous Line Preparation and Chronic Indwelling Catheter Placement
- 05:06Cecal Ligation and Puncture (CLP)
- 07:06Results: Representative Survival Curves after 5 or 7 Days of Critical Illness
- 08:08Conclusion
This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.
