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重组蛋白表达，结晶和生物物理研究<em>芽孢杆菌</em> - 保守的核苷酸焦磷酸化酶，BcMazG

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Biochimica

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JoVE Journal Biochimica

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

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重组蛋白表达，结晶和生物物理研究<em>芽孢杆菌</em> - 保守的核苷酸焦磷酸化酶，BcMazG

DOI:

10.3791/55576-v

•

12:23 min

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May 16, 2017

•

Meong Il Kim, Choongdeok Lee, Minsun Hong

¹Division of Biological Science and Technology,Yonsei University

Capitoli

00:05Titolo
00:55Recombinant Protein Production and Purification of rBC1531
04:47Removal of Affinity Tags by Thrombin Proteolysis
06:32Crystallization Screening and Optimization of rBC1531
07:30Colorimetric NTPase Assay
08:33Results: Purification, Characterization, and Crystallization of rBC1531
10:31Conclusion

Summary

Traduzione automatica

炭疽芽孢杆菌是致命性吸入性炭疽的专性病原体，因此对其基因和蛋白质的研究受到严格管制。另一种方法是研究直系同源基因。我们描述了蜡状芽孢杆菌Bc1531中的炭疽杆菌直系同源物的生物物理化学研究，需要最少的实验设备和缺乏严重的安全性问题。

Tags

Keywords: Recombinant Protein Bacillus Nucleotide Pyrophosphorylase BcMazG Protein Expression Protein Crystallization Biophysical Studies E. Coli Chemically Competent Cells Transformation Plasmid DNA DNA Sequencing Protein Purification IPTG Induction Cell Lysis Protein Characterization

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