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的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入

JoVE Journal
Biologia

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JoVE Journal Biologia

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

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的多孔冷冻管省去冻结工件时肌肉组织在液氮中直接浸入

DOI:

10.3791/55616-v

•

06:42 min

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April 06, 2017

•

Yizhong Huang, Maozhang He, Qingjie Zeng, Lin Li, Zhen Zhang, Junwu Ma, Yanyu Duan

¹State Key Laboratory for Pig Genetic Improvement and Production Technology,Jiangxi Agricultural University

Capitoli

00:05Titolo
01:17Equipment for Sample Collection and Preparation of Muscle Samples
02:47Freezing and Storing Muscles, and Slide Preparation
04:40Results: Freezing Muscle Tissue Using a Multi-hole Cryovial
06:10Conclusion

Summary

Traduzione automatica

这个协议描述的过程通过直接让他们与液氮冷冻的肌肉组织。该协议还突出一个新的离心管，可避免氮气的“毯效应”，当液氮接触样本的组织表面上。

Tags

AI-assisted Freezing Cryogenic Sample Preparation Multi-hole Cryovial Liquid Nitrogen Freezing Freezing Artifact Elimination Cryostat Sectioning

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