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A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
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Biologia
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JoVE Journal Biologia
A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

DOI:
10.3791/55616-v

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06:42 min

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April 06, 2017

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, , , , , ,
1State Key Laboratory for Pig Genetic Improvement and Production Technology,Jiangxi Agricultural University

Capitoli

  • 00:05Titolo
  • 01:17Equipment for Sample Collection and Preparation of Muscle Samples
  • 02:47Freezing and Storing Muscles, and Slide Preparation
  • 04:40Results: Freezing Muscle Tissue Using a Multi-hole Cryovial
  • 06:10Conclusion

Summary

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This protocol describes a procedure to freeze muscle tissues by plunging them directly into liquid nitrogen. This protocol also highlights a new cryovial that can avoid the "blanket effect" of nitrogen gas when liquid nitrogen contacts the tissue surface of a specimen.

Tags

AI-assisted FreezingCryogenic Sample PreparationMulti-hole CryovialLiquid Nitrogen FreezingFreezing Artifact EliminationCryostat Sectioning

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