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Evaluating Primary Blast Effects In Vitro
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Evaluating Primary Blast Effects In Vitro
DOI:

10:51 min

September 18, 2017

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Capitoli

  • 00:05Titolo
  • 01:00Cell Culture and Blast Sample Preparation
  • 02:27Shock Tube Assembly
  • 04:46Shock Tube Operation
  • 07:01Cell Viability Assay
  • 08:52Results: Application of 127 kPa Shock Wave is Detrimental to Cell Growth
  • 09:48Conclusion

Summary

Traduzione automatica

Understanding how cells are modulated by exposure to shock waves can help identify the mechanisms behind injuries triggered from blast events. This protocol uses custom-built shock tube equipment to apply shock waves at a range of pressures to cell monolayers and to identify the subsequent effects on cell viability.

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