Rivista
/
/
二维和三维胚体小鼠胚胎干细胞的维甲酸诱导神经分化的分析
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
DOI:

09:04 min

April 22, 2017

, , ,

Capitoli

  • 00:05Titolo
  • 00:51Embryoid Body Formation
  • 01:582D Embryoid Body Culture and Detection of Secreted Proteins
  • 03:043D Embryoid Body Culture
  • 04:06Embryoid Body Dissociation
  • 05:56Analysis of Embryoid Body Formation by Immunofluorescence
  • 07:12Results: Characteristics of Embryo Body Cultures and Visualization of Neural Differentiation Markers
  • 08:22Conclusion

Summary

Traduzione automatica

我们描述了使用小鼠胚胎干细胞生成二维或三维的胚状体的技术。然后,我们解释如何以诱导胚状体细胞视黄酸,以及如何的神经分化由祖细胞标志物的免疫荧光和免疫印迹来分析其分化状态。

Video correlati

Read Article