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In Ovo Electroporation in the Chicken Auditory Brainstem
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
In Ovo Electroporation in the Chicken Auditory Brainstem

In Ovo Electroporation in the Chicken Auditory Brainstem

DOI:
10.3791/55628-v

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10:14 min

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June 09, 2017

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, ,
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders,Northwestern University, 2Knowles Hearing Research Center,Northwestern University, 3Department of Neurobiology,Northwestern University

Capitoli

  • 00:05Titolo
  • 00:54Plasmid Preparation
  • 02:01Egg Handling and Windowing
  • 04:15Plasmid Injection
  • 06:11Electroporation
  • 08:03Results: Spatial Protein Expression After In Ovo Electroporation
  • 09:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.

Tags

In Ovo ElectroporationChicken Auditory BrainstemGene ExpressionAuditory Neuron DevelopmentPlasmid SolutionMicropipette PullerPicospritzerHamburger-Hamilton StageEgg IncubationEggshell Membrane RemovalAlbumin Withdrawal

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