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在奥运电击鸡听觉脑干中
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
In Ovo Electroporation in the Chicken Auditory Brainstem
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在奥运电击鸡听觉脑干中

DOI:
10.3791/55628-v

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10:14 min

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June 09, 2017

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, ,
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders,Northwestern University, 2Knowles Hearing Research Center,Northwestern University, 3Department of Neurobiology,Northwestern University

Capitoli

  • 00:05Titolo
  • 00:54Plasmid Preparation
  • 02:01Egg Handling and Windowing
  • 04:15Plasmid Injection
  • 06:11Electroporation
  • 08:03Results: Spatial Protein Expression After In Ovo Electroporation
  • 09:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

鸟类和哺乳动物的听觉脑干神经元专门用于快速神经编码，这是正常听力功能的基本过程。这些神经元来自胚胎后脑的不同前体。我们提出利用电穿孔技术在鸡胚后脑中表达基因以研究听觉发育过程中的基因功能。

Tags

Keywords: In Ovo ElectroporationChicken Auditory BrainstemGene ExpressionAuditory Neuron DevelopmentPlasmid SolutionMicropipette PullerPicospritzerHamburger-Hamilton StageEgg IncubationEggshell Membrane RemovalAlbumin Withdrawal

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