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从多个小鼠组织分离中间丝状蛋白以研究与老化相关的翻译后修饰

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

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从多个小鼠组织分离中间丝状蛋白以研究与老化相关的翻译后修饰

DOI:

10.3791/55655-v

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09:29 min

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May 18, 2017

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Rachel A. Battaglia, Parijat Kabiraj, Helen H. Willcockson, Melinda Lian, Natasha T. Snider

¹Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Capitoli

00:05Titolo
00:49Preparation of Detergent-soluble and High-salt Extracts of Tissue-specific IFs
03:16Automated Tissue Lysis for RNA and IF Protein Extraction in High-volume Experiments
07:15Results: High-salt Extraction of Intermediate Filament Proteins Using Lysing Matrix
08:50Conclusion

Summary

Traduzione automatica

在这种方法中，我们提出了从多个小鼠组织快速高效地分离中间丝（IF）蛋白质的生物化学方法。分离的IF可用于通过质谱法和其他生化测定法研究翻译后修饰的变化。

Tags

Keywords: Intermediate Filament Proteins Post-translational Modifications Triton X-100 Buffer High Salt Buffer PBS EDTA Buffer SDS Sample Buffer Centrifugation Homogenization

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