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活细胞成像血小板脱水和分泌流量
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Biologia
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JoVE Journal Biologia
Live-cell Imaging of Platelet Degranulation and Secretion Under Flow
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活细胞成像血小板脱水和分泌流量

DOI:
10.3791/55658-v

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11:42 min

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July 10, 2017

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, , , , ,
1Department of Clinical Chemistry and Haematology,University Medical Center Utrecht, 2Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences,Utrecht University

Capitoli

  • 00:05Titolo
  • 01:12Cover Glass Preparation
  • 02:25Platelet Isolation and Washing
  • 03:58Flow Chamber Assembly
  • 05:08Pre-strained Platelets and Microscope Setup
  • 07:36Microscope Imaging of Platelets
  • 09:31Results: Platelet Degranulation and Secretion Under Flow
  • 10:57Conclusion

Summary

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这项工作描述了基于荧光显微镜的方法研究血小板粘附，扩散和分泌的流动。这种多功能平台能够调查血小板功能，用于血栓形成和止血的机械研究。

Tags

Keywords: Live-cell ImagingPlatelet DegranulationPlatelet SecretionFlowPlatelet BiologyPlatelet IsolationPlatelet PreactivationVon Willebrand FactorFibrinogenHEPES Tyrode BufferPlatelet-rich PlasmaCitrated Whole Blood

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