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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

DOI:
10.3791/55659-v

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15:27 min

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April 17, 2017

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, , ,
1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science,Columbia University

Capitoli

  • 00:05Titolo
  • 01:06Kinetic Measurements in the Biacore T100
  • 03:17Kinetic Measurements in the ProteOn XPR36
  • 06:03Kinetic Measurements in the Octet RED384
  • 08:53Kinetic Measurements in the IBIS MX96
  • 11:07Analysis of High-Affinity Antibody-Antigen Binding Kinetics over Four Biosensor Platforms
  • 12:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.

Tags

Antibody-antigen BindingBiosensor PlatformsKinetic DataDrug DiscoveryBiacore T100ProteOn XPR36Experimental DesignKinetic MeasurementsHigh-affinity InteractionsData ConsistencyOperational Efficiency

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