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Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
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JoVE Journal Genetica
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation

DOI:
10.3791/55689-v

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09:40 min

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June 27, 2017

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, ,
1Department of Gastroenterology, Hepatology and Nutrition,The University of Texas MD Anderson Cancer Center

Capitoli

  • 00:05Titolo
  • 00:41Cell Fixation and Shearing
  • 04:21Immunoprecipitation and Elution
  • 06:42Results: TGF-β1-induced Binding of Transcription Factor SMAD2 to its Putative Binding Site in the c-KIT Promoter
  • 08:37Conclusion

Summary

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DNA-protein interactions are essential for multiple biological processes. During the evaluation of cellular functions, the analysis of DNA-protein interactions is indispensable for understanding gene regulation. Chromatin immunoprecipitation (ChIP) is a powerful tool to analyze such interactions in vivo.

Tags

Keywords: C-KIT Ligand PromoterChromatin ImmunoprecipitationSMAD2TGF Beta 1Transcription FactorGene TranscriptionCell CultureCell FixationCell LysisNuclei DigestionMicrococcal NucleaseChromatin ShearingOptimization

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