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重建神经元电路：快速神经延伸和功能性神经元连接的新方法
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Neuroscienze
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JoVE Journal Neuroscienze
Rewiring Neuronal Circuits: A New Method for Fast Neurite Extension and Functional Neuronal Connection
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重建神经元电路：快速神经延伸和功能性神经元连接的新方法

DOI:
10.3791/55697-v

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10:26 min

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June 13, 2017

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, , , , ,
1Department of Physics,McGill University, 2Department of Neurology and Neurosurgery,Montreal Neurological Institute, 3Ananda Devices

Capitoli

  • 00:05Titolo
  • 01:01Standardization of Neuronal Cultures Using Microfluidic Devices: Device Assambly
  • 02:54Plating Neurons in Microfluidic Systems
  • 04:47PDL-bead Adhesion and Micromanipulation
  • 06:13Pulling Neurites and Connecting Neurons
  • 08:45Results: The Newly Induced, Elongated, and Connected Neurite Can Transfer Information Between Two Isolated Neuronal Populations
  • 09:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

该程序描述了如何使用固定在引导神经突伸长的微量移液管上的聚-D-赖氨酸包被的珠快速启动，延伸和连接在微流体室中组织的神经突。

Tags

Neurite ExtensionFunctional Neuronal ConnectionNeurite Growth RateSynapse FormationNeural NetworkSingle-cell ManipulationExperimental ReproducibilityPDL CoatingMicrofluidic DevicesCell CultureNeuroscienze

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