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Biologia dello sviluppo

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Dissection and Culture of Mouse Embryonic Kidney

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Dissection and Culture of Mouse Embryonic Kidney

Dissection and Culture of Mouse Embryonic Kidney

DOI:

10.3791/55715-v

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08:30 min

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May 17, 2017

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Bejan Aresh, Christiane Peuckert

¹Department of Neuroscience,Uppsala University, ²Department of Organismal Biology, Evolutionary Biology Center,Uppsala University

Capitoli

00:05Titolo
00:53Metanephric Rudiment Dissection at E11.5
04:04Metanephric Anlagen Fixation and Staining
06:07Results: Representative Ureteric Bud Branching Analyses
07:48Conclusion

Summary

Traduzione automatica

This protocol describes a method for isolating and culturing metanephric rudiments from mouse embryos.

Tags

Mouse Embryonic Kidney Dissection Culture Metanephric Rudiments Ureteric Bud Branching Morphology Kidney Organogenesis Tubular Branching Morphogenesis Epithelial Mesenchyme Interactions Congenital Malformations Polycarbonate Membrane Filter Embryonic Day 11 1/2 Forceps Dorsal Aorta Ventral Body Wall Kidney Anlagen

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