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DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
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Biologia
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JoVE Journal Biologia
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum

DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum

DOI:
10.3791/55740-v

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06:05 min

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June 16, 2017

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, , , ,
1Department of Orthopedic Surgery,Harvard Medical School and Brigham and Women’s Hospital, 2Division of Graduate Medical Sciences,Boston University

Capitoli

  • 00:05Titolo
  • 00:50Axolotl Chest Opening
  • 02:04Axolotl Perfusion and Vasculature Visualization
  • 04:34Results: Representative Fully Perfused Axolotl Imaging
  • 04:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

Using a lipophilic 1,1'-Dioctadecy-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) staining technique, Ambystoma mexicanum can undergo vascular perfusion to allow for easy visualization of the vasculature.

Tags

Dil PerfusionVascular VisualizationAmbystoma MexicanumRegenerative MedicineAxolotlLimb RegenerationSedationThoracic CavityPericardiumAortaPeristaltic PumpPBSPerfusion

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