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从骨髓间充质干细胞制备血浆膜囊泡用于潜在的细胞质替代治疗

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Preparation of Plasma Membrane Vesicles from Bone Marrow Mesenchymal Stem Cells for Potential Cytoplasm Replacement Therapy

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从骨髓间充质干细胞制备血浆膜囊泡用于潜在的细胞质替代治疗

DOI:

10.3791/55741-v

•

09:34 min

•

May 18, 2017

•

Li-qun Xu, Mei-jia Lin, Yun-pan Li, Shuang Li, Shao-jun Chen, Chi-ju Wei

¹Multidisciplinary Research Center,Shantou University

Capitoli

00:05Titolo
01:10Plasma Membrane Vesicle (PMV) Generation
03:18PMV Content Examination by Confocal Microscopy
05:11PMV Gastrocnemius Muscle Injection
07:12Results: Representative PMV Imaging and Analyses
08:40Conclusion

Summary

Traduzione automatica

与年龄相关的疾病与细胞质组分中的多种缺陷有关。在这里，我们提出了从骨髓间充质干细胞制备质膜囊泡的方案。这种技术可能被用作细胞质替代疗法的手段，以改善或甚至逆转年龄相关表型。

Tags

Plasma Membrane Vesicles PMVs Bone Marrow Mesenchymal Stem Cells BMSCs Cytoplasm Replacement Therapy Mechanical Extrusion Cell Culture Trypsin-EDTA Phase Contrast Microscopy Mitochondrial Membrane Potential

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