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In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
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JoVE Journal Biologia dello sviluppo
In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

DOI:
10.3791/55757-v

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09:05 min

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August 09, 2017

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, , ,
1Create Fertility Centre, 2Department of Physiology,University of Toronto, 3Department of Obstetrics and Gynecology,University of Toronto, 4Department of Physiology,University of Toronto, 5Department of Obstetrics and Gynecology,Women’s College Hospital

Capitoli

  • 00:05Titolo
  • 00:58Preparation of Primary Rat Cardiac, Human MSC Co-cultures
  • 03:55Preparation of Aggregate Co-cultures
  • 05:43Results: Differentiation of Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
  • 07:53Conclusion

Summary

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Here, we present a method to efficiently harness the cardiac differentiation potential of young sources of human mesenchymal stem cells in order to generate functional, contracting, cardiomyocyte-like cells in vitro.

Tags

Keywords: Mesenchymal Stem CellsCardiomyocyte DifferentiationIn Vitro DifferentiationCardiac Fetal LayersCardiovascular DiseaseTrypsin DigestionDMEM-F12MSC CulturesCell Dissociation

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