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Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
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JoVE Journal Neuroscienze
Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

DOI:
10.3791/55770-v

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08:32 min

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July 13, 2017

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, , , , , , , , , , , , ,
1Neuroregeneration Laboratory, Department of Anesthesiology,University of California, San Diego, 2Institute of Animal Physiology and Genetics,Czech Academy of Sciences, 3Department of Neurosurgery,University of California, San Diego, 4Institute of Neurobiology,Slovak Academy of Sciences

Capitoli

  • 00:05Titolo
  • 01:19Exposing the Pial Membrane
  • 03:58Opening the Pial Membrane and Insering the Subpial Needle for AAV9 Delivery
  • 06:11Results: GFP-expression in Spine Parenchyma and Brain Following Subpial AAV9 Delivery
  • 07:52Conclusion

Summary

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The goal of the present study was to develop and validate the potency and safety of spinal adeno-associated virus 9 (AAV9)-mediated gene delivery by using a novel subpial gene delivery technique in adult mice.

Tags

SubpialAAV9Vector DeliverySpinal CordSpinal Gene ExpressionSpinal Neurodegenerative DisordersAmyotrophic Lateral SclerosisSpinal Traumatic InjuryChronic PainTransgene ExpressionSpinal Gray MatterSpinal White MatterSpinal SegmentsDura-openingSubpial NeedleAnesthesiaIsofluraneParavertebral MuscleSpinal LaminectomyDura IncisionCervicalOccipital Bone

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