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在成年小鼠中的亚型腺相关病毒9（AAV9）载体递送

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

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在成年小鼠中的亚型腺相关病毒9（AAV9）载体递送

DOI:

10.3791/55770-v

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08:32 min

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July 13, 2017

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Takahiro Tadokoro, Atsushi Miyanohara, Michael Navarro, Kota Kamizato, Stefan Juhas, Jana Juhasova, Silvia Marsala, Oleksandr Platoshyn, Erik Curtis, Brandon Gabel, Joseph Ciacci, Nada Lukacova, Katarina Bimbova, Martin Marsala⁴

¹Neuroregeneration Laboratory, Department of Anesthesiology,University of California, San Diego, ²Institute of Animal Physiology and Genetics,Czech Academy of Sciences, ³Department of Neurosurgery,University of California, San Diego, ⁴Institute of Neurobiology,Slovak Academy of Sciences

Capitoli

00:05Titolo
01:19Exposing the Pial Membrane
03:58Opening the Pial Membrane and Insering the Subpial Needle for AAV9 Delivery
06:11Results: GFP-expression in Spine Parenchyma and Brain Following Subpial AAV9 Delivery
07:52Conclusion

Summary

Traduzione automatica

本研究的目的是通过在成年小鼠中使用新的唾液基因传递技术来开发和验证脊髓腺相关病毒9（AAV9）介导的基因递送的效力和安全性。

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