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Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry
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JoVE Journal Immunologia e infezioni
Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

Analysis of Microglia and Monocyte-derived Macrophages from the Central Nervous System by Flow Cytometry

DOI:
10.3791/55781-v

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10:43 min

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June 22, 2017

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, , ,
1Inserm U 1227, CNRS UMR 7225, 2Sorbonne Universités,UPMC, University of Paris, 3Institut du Cerveau et de la Moelle épinière, ICM, 4AP-HP, Hôpital de la Pitié Salpêtrière;

Capitoli

  • 00:05Titolo
  • 00:52Perfusion and Brain and Spinal Cord Dissection
  • 03:18Cell Dissociation and Density Gradient Separation
  • 06:00Cell Labeling for Flow Cytometry
  • 08:06Results: Representative Brain and Spinal Cord Macrophage Subpopulation Analysis
  • 09:31Conclusion

Summary

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This protocol provides an analysis of the macrophage subpopulations in the adult mouse central nervous system by flow cytometry and is helpful for the study of multiple markers expressed by these cells.

Tags

Keywords: MicrogliaMonocyte-derived MacrophagesCentral Nervous SystemFlow CytometryNeuroimmunologyMacrophage SubpopulationsCell Marker AnalysisCell IsolationSpinal CordBrain

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