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Bioengineering

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JoVE Journal Bioengineering
Isolatie van Primaire Murine Retinale Ganglioncellen (RGC's) door Flow Cytometry
 
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Isolatie van Primaire Murine Retinale Ganglioncellen (RGC's) door Flow Cytometry

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Miljoenen mensen lijden aan retinale degeneratieve ziekten die leiden tot onomkeerbare blindheid. Een gemeenschappelijk element van veel van deze ziekten is het verlies van retinale ganglioncellen (RGC's). Dit gedetailleerde protocol beschrijft de isolatie van primaire murine RGC's door positieve en negatieve selectie met flowcytometrie.

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Bioengineering nummer 125 retinale ganglioncellen RGC flowcytometrie neurodegeneratie glaucoom veroudering
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