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Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
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JoVE Journal Medicina
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

DOI:
10.3791/55797-v

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11:00 min

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September 18, 2017

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, , , , , ,
1Department of Cardiology, Sir Run Run Shaw Hospital,Zhejiang University School of Medicine, 2Experimental Research Center,China Academy of Chinese Medical Sciences, 3Department of Cardiology,The Third Hospital of Hangzhou City

Capitoli

  • 00:05Titolo
  • 00:44Isolation of Left Ventricular Cardiomyocytes
  • 04:15Set Up of Perfusion System
  • 04:59Measurement of Intracellular Ca2+ Transient and Sarcomere Shortening
  • 06:51Assessment of SR Ca2+ Reserve and Diastolic Ca2+ Removal Function
  • 09:10Results: Assessment of SR Calcium Reserve and Calcium Removal Function
  • 10:17Conclusion

Summary

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Intracellular calcium recycling plays a critical role in regulation of systolic and diastolic function in cardiomyocytes. Here, we describe a protocol to evaluate sarcoplasmic reticulum Ca2+ reserve and diastolic calcium removal function in cardiomyocytes by a calcium imaging system.

Tags

Keywords: Sarcoplasmic ReticulumCalcium ReserveIntracellular Diastolic Calcium RemovalIsolated Ventricular CardiomyocytesLangendorff PerfusionNT SolutionCalcium-free Tyrode SolutionCollagenase AKB SolutionCell IsolationCell FiltrationCentrifugation

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