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肌网钙储备与细胞内舒张钙在心室肌细胞中的分离评价
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Medicina
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JoVE Journal Medicina
Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes
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肌网钙储备与细胞内舒张钙在心室肌细胞中的分离评价

DOI:
10.3791/55797-v

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11:00 min

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September 18, 2017

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, , , , , ,
1Department of Cardiology, Sir Run Run Shaw Hospital,Zhejiang University School of Medicine, 2Experimental Research Center,China Academy of Chinese Medical Sciences, 3Department of Cardiology,The Third Hospital of Hangzhou City

Capitoli

  • 00:05Titolo
  • 00:44Isolation of Left Ventricular Cardiomyocytes
  • 04:15Set Up of Perfusion System
  • 04:59Measurement of Intracellular Ca2+ Transient and Sarcomere Shortening
  • 06:51Assessment of SR Ca2+ Reserve and Diastolic Ca2+ Removal Function
  • 09:10Results: Assessment of SR Calcium Reserve and Calcium Removal Function
  • 10:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

细胞内钙循环对心肌细胞收缩和舒张功能的调节起着至关重要的作用。在这里, 我们描述了一个协议, 以评估肌网钙2 +储备和舒张钙功能的心肌细胞钙显像系统。

Tags

Sarcoplasmic ReticulumCalcium ReserveIntracellular Diastolic Calcium RemovalIsolated Ventricular CardiomyocytesLangendorff PerfusionNT SolutionCalcium-free Tyrode SolutionCollagenase AKB SolutionCell IsolationCell FiltrationCentrifugation

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