/

/

通过从光响应聚合物纳米载体的siRNA释放的时空控制预测基因沉默

JoVE Journal
Bioingegneria

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Bioingegneria

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Please note that all translations are automatically generated. Click here for the English version.

通过从光响应聚合物纳米载体的siRNA释放的时空控制预测基因沉默

DOI:

10.3791/55803-v

•

11:53 min

•

July 21, 2017

•

Chad T. Greco, Thomas H. Epps, III², Millicent O. Sullivan

¹Department of Chemical and Biomolecular Engineering,University of Delaware, ²Department of Materials Science and Engineering,University of Delaware

Capitoli

00:05Titolo
00:56Formulation of siRNA Nanocarriers
02:09Measuring siRNA Release Using Gell Electrophoresis
04:57Measuring siRNA Release Using Fluorescence Correlation Spectroscopy (FCS)
06:12Cell Culture and In Vitro siRNA Delivery
08:15Controlling Gene Silencing in a Spatiotemporal Manner
09:35Results: Prediction and Spatiotemporal Control of Gene Silencing
11:07Conclusion

Summary

Traduzione automatica

我们提出一种新的方法，使用光响应嵌段共聚物更有效的时空控制基因沉默，没有可检测的脱靶效应。此外，可以使用简单的siRNA释放测定法和简单的动力学建模来预测基因表达的变化。

Tags

Gene Silencing SiRNA Release Photo-responsive Polymeric Nanocarriers Spatiotemporal Control Structure-function Relationships Stimuli-responsive Delivery Kinetic Modeling Gene Expression Control Photo-responsive Polymers N/P Ratio SDS Solution UV Laser Nanocarrier Solution

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

制定通过Nanoprecipitation技术嵌段聚合物纳米粒子

组合合成和高通量的蛋白质释放的聚合物薄膜和纳米粒子库

工程双层凝胶控制升序分化

高分子基因传递纳米粒子的纳米粒子跟踪分析和高通量流式细胞仪评价

的使用<em>前体内</em>钱德勒循环装置，以评估改性聚合物血导管的生物相容性

多孔硅微粒进行交付的siRNA治疗的

聚合物微针阵列制造用光刻

在脉冲激光激发金纳米粒子集成光电响应脂质体及其微泡空化的测量的合成

治疗的siRNA递送至CNS使用阳离子和阴离子脂质体

闪速纳米沉淀法包聚物纳米粒子中疏水亲水化合物的作用

Read Article