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Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
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Capitoli
- 00:05Titolo
- 01:06Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
- 04:12Embryo Dechorionation
- 05:15Preparation of Embryo Extract
- 07:15Results: Preparation of Drosophila Embryo Extracts for Proteomics Experiments
- 08:35Conclusion
The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).
