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中等规模的制备<em>果蝇</em>蛋白质组学实验胚胎提取物

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

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中等规模的制备<em>果蝇</em>蛋白质组学实验胚胎提取物

DOI:

10.3791/55804-v

•

09:16 min

•

May 30, 2017

•

Liu Yang, Sayantanee Paul, Sarah DuBois-Coyne, Phillip Kyriakakis, Alexey Veraksa

¹Biology Department,University of Massachusetts Boston, ²Department of Bioengineering,University of California, San Diego

Capitoli

00:05Titolo
01:06Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
04:12Embryo Dechorionation
05:15Preparation of Embryo Extract
07:15Results: Preparation of Drosophila Embryo Extracts for Proteomics Experiments
08:35Conclusion

Summary

Traduzione automatica

该方案的目标是提供一种直接和廉价的方法来收集中等规模的果蝇胚胎（0.5-1 g），并制备可用于下游蛋白质组学应用的蛋白质提取物，如亲和纯化 - 质谱（AP-MS ）。

Tags

Drosophila Embryo Proteomics Protein Extraction Affinity Purification Mass Spectrometry Cell Signaling Biologia dello sviluppo Medium-scale User-friendly Fly Cage Apple Juice Plates Yeast Paste

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