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Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
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JoVE Journal Biologia
Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

DOI:
10.3791/55809-v

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07:28 min

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June 19, 2017

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, , , , ,
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Capitoli

  • 00:05Titolo
  • 00:52Whitefly Rearing, Cryptic Species Identification, and Endosymbiont Identification and Localization
  • 02:11Whitefly Bacteriome Dissection and Purification
  • 03:47Amplification of Endosymbiont Genomes
  • 05:02Results: Extraction and Genome Sequencing of Endosymbionts from the Whitefly Bemisia tabaci
  • 06:12Conclusion

Summary

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Here, we present a protocol to isolate endosymbionts from the whitefly Bemisia tabaci through dissection and filtration. After amplification, the DNA samples are suitable for subsequent sequencing and study of the mutualism between endosymbionts and whitefly.

Tags

Keywords: EndosymbiontsWhitefly Bemisia TabaciPCRDNA ExtractionBacteriome DissectionInsect EndosymbiontsBacterial Isolation

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